Discover the principles behind Countable PCR, how it compares with established molecular quantification methods, and what direct RNA counting could mean for research and clinical applications.

CRISPR genome editing generates double-stranded breaks (DSBs) in genomic DNA and is a targeted method by which to achieve gene knockouts and knock-ins. Endogenous repair of DSBs results in an array of ...

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

Scientists from the United States have recently identified a novel approach to rapidly and accurately detect the omicron BA.2.12.1 sub-lineage of severe acute respiratory syndrome coronavirus 2 ...

In this Innovation Spotlight, Marwan A. Alsarraj, global segment manager at Bio-Rad Laboratories, discusses how digital PCR eliminates the need for standard curves while achieving high sensitivity and ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

A new form of PCR has been used to improve the library preparation for forensic DNA typing from difficult samples like touch evidence and human remains. Typically, DNA sequencing and analysis is ...

Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...

Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a variety of methods, including techniques that utilize ...