What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle. For quantitative reverse transcription PCR ...

Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of ...

In a recent study posted to the medRxiv*preprint server, a team of researchers from Israel developed and validated novel reverse transcriptase - quantitative polymerase chain reaction (RT-qPCR) assays ...

1. To minimize the potential for sample cross-contamination and nucleic acid carryover from one experiment to the next, use aerosol-resistant pipette tips and designated work areas and pipettes for ...

This 5-day training is designed to provide a solid understanding of specific topics through presentation and laboratory work. Participants will gain significant experience in the performance of ...

MADISON, Wis.--(BUSINESS WIRE)--Promega Corporation’s GoTaq® Probe 1-Step RT-qPCR System is now in a Centers for Disease Control and Prevention COVID-19 diagnostic protocol for emergency use. The ...